THREONINE DEAMINATION IN ESCHERICHIA COLI II
نویسندگان
چکیده
منابع مشابه
Deamination of serine. II. D-Serine dehydrase, a vitamin B6 enzyme from Escherichia coli.
Non-enzymatic deamination of serine and cysteine is catalyzed by pyridoxal and certain metal salts at 100” (2). This finding suggested that pyridoxal phosphate might be involved in the enzymatic deamination of these amino acids. Vitamin B, has already been implicated in the desulfhydration of cysteine by rat liver (3) and of cysteine and homocysteine by bacteria (4). Several similarities of cys...
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Studies were carried out on the formation of biodegradative L-threonine deaminase (EC 4.2.1.16) in resting cells of Escherichia coli. The results obtained are as follows: (1) The enzyme level was increased by adding L-threonine together with yeast extract to the cell suspension. (2) In the course of the enzyme formation, the addition of chloramphenicol or glucose to the medium resulted in insta...
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Two auxotrophic mutants of Escherichia coli have been shown to accumulate significant amounts of l-threonine in the culture medium. One mutant, 13071, is deficient in alpha,epsilon-diaminopimelic acid (DAP), and the other, 13070, is deficient in both DAP and methionine. Accumulation of l-threonine by 13071 in a synthetic medium with mannitol as the carbon source at 37 C incubation temperature w...
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Two distinctly different L-threonine deaminases are known to be formed in Escherichia coli. One of them participates in the biosynthesis of L-isoleucine from L-threonine and is susceptible to an end product inhibition by the former compound (1). The other one seems to participate in the catabolism of L-threonine under anaerobic conditions and is activated by adenosine 5’monophosphate (1, 2). Li...
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Amino-acid producers have traditionally been developed by repeated random mutagenesis owing to the difficulty in rationally engineering the complex and highly regulated metabolic network. Here, we report the development of the genetically defined L-threonine overproducing Escherichia coli strain by systems metabolic engineering. Feedback inhibitions of aspartokinase I and III (encoded by thrA a...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1957
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.73.1.105-112.1957